Open AccessAutomated On-Line Solid-Phase Extraction Coupled with HPLC for Measurement of 5-Hydroxyindole-3-acetic Acid in Urine
Author(s) -
Erik J. Mulder,
Alida Oosterloo-Duinkerken,
George M. Anderson,
Elisabeth G.E. de Vries,
Ruud B. Minderaa,
Ido P. Kema
Publication year - 2005
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2005.050062
Subject(s) - chromatography , urine , solid phase extraction , high performance liquid chromatography , analyte , chemistry , elution , extraction (chemistry) , acetic acid , urinary system , excretion , detection limit , medicine , biochemistry
Quantification of 5-hydroxyindole-3-acetic acid (5-HIAA) in urine is useful in diagnosing and monitoring of patients with carcinoid tumors and in the study of serotonin (5-hydroxytryptamine) metabolism in various disorders. We describe an automated method that incorporates on-line solid-phase extraction (SPE) and HPLC to measure urinary 5-HIAA.
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