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High-Throughput Purification of Viral RNA Based on Novel Aqueous Chemistry for Nucleic Acid Isolation
Author(s) -
M. K. Hourfar,
Uwe Michelsen,
Michael Schmidt,
Annemarie Berger,
Erhard Seifried,
W. Kurt Roth
Publication year - 2005
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2005.048603
Subject(s) - nucleic acid , detection limit , chromatography , chaotropic agent , chemistry , extraction (chemistry) , nucleic acid methods , solid phase extraction , elution , aqueous solution , rna extraction , analyte , rna , biochemistry , organic chemistry , gene
Extraction protocols using magnetic solid phases offer a high potential for automation. However, commercially available magnetic-bead-based assays either lack the sensitivity required for viral diagnostics or are disproportionately expensive.

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