CYP2D6 Genotyping by a Multiplex Primer Extension Reaction | Zendy

Johanna Sistonen | Zendy; Silvia Fuselli | Zendy; Antti Levo | Zendy; Antti Sajantila | Zendy

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CYP2D6 Genotyping by a Multiplex Primer Extension Reaction

Author(s) -

Johanna Sistonen,

Silvia Fuselli,

Antti Levo,

Antti Sajantila

Publication year - 2005

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2004.046466

Subject(s) - genotyping , multiplex , primer (cosmetics) , primer extension , multiplex polymerase chain reaction , computational biology , genetics , biology , genotype , polymerase chain reaction , chemistry , base sequence , dna , gene , organic chemistry

we designed a cost-effective and technically\udfeasible CYP2D6 genotyping technique through a\ud2-step assay that provides a straightforward interpretation of results. It allows detection of 11 of the most relevant polymorphic positions, the assessment of wholegene deletion and duplication, and unlike most available typing methods, the allele composition of the gene duplication

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