Preparation and Characterization of Candidate Reference Materials for Telomerase Assays | Zendy

John P. Jakupciak | Zendy; Peter E. Barker | Zendy; Wendy Wang | Zendy; Sudhir Srivastava | Zendy; Donald H. Atha | Zendy

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Preparation and Characterization of Candidate Reference Materials for Telomerase Assays

Author(s) -

John P. Jakupciak,

Peter E. Barker,

Wendy Wang,

Sudhir Srivastava,

Donald H. Atha

Publication year - 2005

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2004.044727

Subject(s) - telomerase , telomerase reverse transcriptase , microbiology and biotechnology , chemistry , reverse transcriptase , telomere , real time polymerase chain reaction , biology , polymerase chain reaction , chromatography , biochemistry , dna , gene

Telomerase has been measured in body fluids of cancer patients, and clinical tests for telomerase may have utility as noninvasive, cost-effective methods for the early detection of cancer. However, telomerase activity measured by common methods such as the telomerase repeat amplification protocol (TRAP) and telomerase reverse transcriptase catalytic subunit (hTERT) mRNA by reverse transcription-PCR (RT-PCR) varies among laboratories.

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