Evaluation of a Candidate Reference Measurement Procedure for Serum Free Testosterone Based on Ultrafiltration and Isotope Dilution–Gas Chromatography–Mass Spectrometry | Zendy

Katleen Van Uytfanghe | Zendy; Dietmar Stöckl | Zendy; JeanMarc Kaufman | Zendy; Tom Fiers | Zendy; H A Ross | Zendy; A.P. De Leenheer | Zendy; Linda M. Thienpont | Zendy

Open Access

Evaluation of a Candidate Reference Measurement Procedure for Serum Free Testosterone Based on Ultrafiltration and Isotope Dilution–Gas Chromatography–Mass Spectrometry

Author(s) -

Katleen Van Uytfanghe,

Dietmar Stöckl,

JeanMarc Kaufman,

Tom Fiers,

H A Ross,

A.P. De Leenheer,

Linda M. Thienpont

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2004.037358

Subject(s) - chromatography , isotope dilution , ultrafiltration (renal) , chemistry , mass spectrometry , gas chromatography–mass spectrometry , gas chromatography , free fraction , analytical chemistry (journal) , plasma protein binding , biochemistry

To assess the analytical validity of free testosterone (FTe) measurements, a reference measurement procedure (RMP) is required. For steroids, isotope dilution-mass spectrometry is accepted as state-of-the-art technology. Because FTe is defined as the hormone fraction in serum water in equilibrium with the protein-bound fraction, the RMP should include a physical separation step. The use of equilibrium dialysis (ED) or ultrafiltration (UF) is advocated. Our objective was to develop such a candidate RMP.

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