Validation of RNA Arbitrarily Primed PCR Probes Hybridized to Glass cDNA Microarrays: Application to the Analysis of Limited Samples | Zendy

Mònica Grau | Zendy; Xavier Solé | Zendy; Antònia ObradorHevia | Zendy; Gemma Tarafa | Zendy; Elisenda Vendrell | Zendy; Joan Valls | Zendy; Vı́ctor Moreno | Zendy; Miguel A. Peinado | Zendy; Gabriel Capellá | Zendy

Open Access

Validation of RNA Arbitrarily Primed PCR Probes Hybridized to Glass cDNA Microarrays: Application to the Analysis of Limited Samples

Author(s) -

Mònica Grau,

Xavier Solé,

Antònia ObradorHevia,

Gemma Tarafa,

Elisenda Vendrell,

Joan Valls,

Vı́ctor Moreno,

Miguel A. Peinado,

Gabriel Capellá

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2004.036236

Subject(s) - transcriptome , biology , dna microarray , complementary dna , microarray , microbiology and biotechnology , microarray analysis techniques , gene expression profiling , polymerase chain reaction , rna , computational biology , gene expression , gene chip analysis , gene , genetics

The applicability of microarray-based transcriptome massive analysis is often limited by the need for large amounts of high-quality RNA. RNA arbitrarily primed PCR (RAP-PCR) is an unbiased fingerprinting PCR technique that reduces both the amount of initial material needed and the complexity of the transcriptome. The aim of this study was to evaluate the feasibility of using hybridization of RAP-PCR products as transcriptome representations to analyze differential gene expression in a microarray platform.

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