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Detection of Anti-SSA Antibodies by Indirect Immunofluorescence
Author(s) -
Xavier Bossuyt,
Johan Frans,
Ann Hendrickx,
Godelieve Godefridis,
René Westhovens,
Godelieve Mariën
Publication year - 2004
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2004.035964
Subject(s) - antibody , anti nuclear antibody , immunofluorescence , staining , biology , extractable nuclear antigens , microbiology and biotechnology , antigen , anti dsdna antibodies , autoantibody , population , western blot , immunology , pathology , medicine , biochemistry , environmental health , gene
HEp-2 cells that overexpress the human 60-kDa SSA antigen have been used to improve sensitivity and specificity for the detection of anti-SSA antibodies by indirect immunofluorescence. We describe a survey on the detection of anti-SSA antibodies using a commercial substrate that overexpresses SSA.

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