Single-Tube Method for Nucleic Acid Extraction, Amplification, Purification, and Sequencing | Zendy

Rebecca L. Margraf | Zendy; Sam Page | Zendy; Maria Erali | Zendy; Carl T. Wittwer | Zendy

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Single-Tube Method for Nucleic Acid Extraction, Amplification, Purification, and Sequencing

Author(s) -

Rebecca L. Margraf,

Sam Page,

Maria Erali,

Carl T. Wittwer

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2004.035808

Subject(s) - genotyping , nucleic acid , amplicon , genotype , biology , rna extraction , rna , dna extraction , reverse transcriptase , microbiology and biotechnology , polymerase chain reaction , virology , chromatography , gene , genetics , chemistry

The hepatitis C virus (HCV) genotype determines patient prognosis and duration of treatment, but sequencing of the gene is lengthy and labor-intensive. We used a commercially available nucleic acid extraction system to develop a single-tube extraction-to-sequencing (STETS) method for HCV genotyping.

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