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High-resolution melting of PCR amplicons with the DNA dye LCGreen I was recently introduced as a homogeneous, closed-tube method of genotyping that does not require probes or real-time PCR. We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (&lt;/=50 bp).

Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons