Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons | Zendy

Michael Liew | Zendy; Robert J. Pryor | Zendy; Robert Palais | Zendy; Cindy Meadows | Zendy; Maria Erali | Zendy; Elaine Lyon | Zendy; Carl T. Wittwer | Zendy

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Genotyping of Single-Nucleotide Polymorphisms by High-Resolution Melting of Small Amplicons

Author(s) -

Michael Liew,

Robert J. Pryor,

Robert Palais,

Cindy Meadows,

Maria Erali,

Elaine Lyon,

Carl T. Wittwer

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2004.032136

Subject(s) - amplicon , single nucleotide polymorphism , melting curve analysis , genotyping , genotype , high resolution melt , microbiology and biotechnology , biology , genetics , methylenetetrahydrofolate reductase , snp genotyping , polymerase chain reaction , gene

High-resolution melting of PCR amplicons with the DNA dye LCGreen I was recently introduced as a homogeneous, closed-tube method of genotyping that does not require probes or real-time PCR. We adapted this system to genotype single-nucleotide polymorphisms (SNPs) after rapid-cycle PCR (12 min) of small amplicons (</=50 bp).

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