Determination of Folate Vitamers in Human Serum by Stable-Isotope-Dilution Tandem Mass Spectrometry and Comparison with Radioassay and Microbiologic Assay | Zendy

Christine M Pfeiffer | Zendy; Zia Fazili | Zendy; Les McCoy | Zendy; Ming Zhang | Zendy; Elaine W. Gunter | Zendy

Open Access

Determination of Folate Vitamers in Human Serum by Stable-Isotope-Dilution Tandem Mass Spectrometry and Comparison with Radioassay and Microbiologic Assay

Author(s) -

Christine M Pfeiffer,

Zia Fazili,

Les McCoy,

Ming Zhang,

Elaine W. Gunter

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.026955

Subject(s) - chromatography , chemistry , isotope dilution , electrospray ionization , tandem mass spectrometry , extraction (chemistry) , acetic acid , selected reaction monitoring , mass spectrometry , solid phase extraction , liquid chromatography–mass spectrometry , high performance liquid chromatography , biochemistry

Current clinical methods for folate give different results and cannot measure the various forms of folate. We developed an isotope-dilution tandem mass spectrometric method coupled to liquid chromatography (LC/MS/MS) as a candidate reference method for 5-methyltetrahydrofolic acid (5MeTHF), 5-formyltetrahydrofolic acid (5FoTHF), and folic acid (FA) in human serum.

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