Rapid Detection and Differentiation of Human Pathogenic Orthopox Viruses by a Fluorescence Resonance Energy Transfer Real-Time PCR Assay | Zendy

Marcus Panning | Zendy; Marcel Asper | Zendy; Stefanie Kramme | Zendy; Herbert Schmitz | Zendy; Christian Drosten | Zendy

Open Access

Rapid Detection and Differentiation of Human Pathogenic Orthopox Viruses by a Fluorescence Resonance Energy Transfer Real-Time PCR Assay

Author(s) -

Marcus Panning,

Marcel Asper,

Stefanie Kramme,

Herbert Schmitz,

Christian Drosten

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.026781

Subject(s) - virology , real time polymerase chain reaction , virus , biology , vaccinia , variola virus , orthopoxvirus , multiplex , polymerase chain reaction , microbiology and biotechnology , gene , genetics , recombinant dna

The orthopox viruses that are pathogenic for humans include variola major virus (VAR), monkeypox virus (MPV), cowpox virus (CPV), and to a lesser extent, camelpox virus (CML) and vaccinia virus (VAC). PCR is a powerful tool to detect and differentiate orthopox viruses, and real-time PCR has the further advantages of rapid turnaround time, low risk of contamination, capability of strain differentiation, and use of multiplexed probes.

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