Properties of the Reverse Transcription Reaction in mRNA Quantification | Zendy

Anders Ståhlberg | Zendy; Joakim Håkansson | Zendy; Xiaojie Xian | Zendy; Henrik Semb | Zendy; Mikael Kubista | Zendy

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Properties of the Reverse Transcription Reaction in mRNA Quantification

Author(s) -

Anders Ståhlberg,

Joakim Håkansson,

Xiaojie Xian,

Henrik Semb,

Mikael Kubista

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.026161

Subject(s) - reverse transcriptase , reverse transcription polymerase chain reaction , microbiology and biotechnology , gene expression , transcription (linguistics) , biology , gene , complementary dna , general transcription factor , transcription factor ii d , chemistry , rna , promoter , genetics , linguistics , philosophy

In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression.

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