Rapid Detection of K-ras Mutations in Bile by Peptide Nucleic Acid-mediated PCR Clamping and Melting Curve Analysis: Comparison with Restriction Fragment Length Polymorphism Analysis | Zendy

Chiung-Yu Chen | Zendy; ShuChu Shiesh | Zendy; Sheu-Jen Wu | Zendy

Open Access

Rapid Detection of K-ras Mutations in Bile by Peptide Nucleic Acid-mediated PCR Clamping and Melting Curve Analysis: Comparison with Restriction Fragment Length Polymorphism Analysis

Author(s) -

Chiung-Yu Chen,

ShuChu Shiesh,

Sheu-Jen Wu

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.024505

Subject(s) - microbiology and biotechnology , restriction fragment length polymorphism , biology , melting curve analysis , mutant , peptide nucleic acid , gene , real time polymerase chain reaction , point mutation , dna , polymerase chain reaction , genetics

Current methods for detection of K-ras gene mutations are time-consuming. We aimed to develop a one-step PCR technique using fluorescent hybridization probes and competing peptide nucleic acid oligomers to detect K-ras mutations in bile and to compare the efficacy with restriction fragment length polymorphism (RFLP) analysis.

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