Interlaboratory Comparison of Fetal Male DNA Detection from Common Maternal Plasma Samples by Real-Time PCR | Zendy

Kirby L. Johnson | Zendy; Kimberly A. Dukes | Zendy; John Vidaver | Zendy; Erik S LeShane | Zendy; Idania Ramirez | Zendy; William Weber | Zendy; Farideh Z. Bischoff | Zendy; Sinuhe Hahn | Zendy; Arun K. Sharma | Zendy; Dianne Dang | Zendy; Lisa M Hire | Zendy; Diana W. Bianchi | Zendy; Joe Leigh Simpson | Zendy; Wolfgang Holzgreve | Zendy; Sherman Elias | Zendy; K. Klinger | Zendy

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Interlaboratory Comparison of Fetal Male DNA Detection from Common Maternal Plasma Samples by Real-Time PCR

Author(s) -

Kirby L. Johnson,

Kimberly A. Dukes,

John Vidaver,

Erik S LeShane,

Idania Ramirez,

William Weber,

Farideh Z. Bischoff,

Sinuhe Hahn,

Arun K. Sharma,

Dianne Dang,

Lisa M Hire,

Diana W. Bianchi,

Joe Leigh Simpson,

Wolfgang Holzgreve,

Sherman Elias,

K. Klinger

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.024380

Subject(s) - testis determining factor , concordance , fetus , polymerase chain reaction , biology , dna , andrology , genomic dna , prenatal diagnosis , microbiology and biotechnology , pregnancy , genetics , medicine , y chromosome , gene

Analysis of fetal DNA from maternal plasma by PCR offers great potential for noninvasive prenatal genetic diagnosis. To further evaluate this potential, we developed and validated a standard protocol to determine whether fetal DNA sequences could be reproducibly amplified and measured across multiple laboratories in a common set of specimens.

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