Validation of a Rapid and Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for Free and Total Mycophenolic Acid | Zendy

Frank Streit | Zendy; Maria Shipkova | Zendy; Victor W. Armstrong | Zendy; Michael Oellerich | Zendy

Open Access

Validation of a Rapid and Sensitive Liquid Chromatography–Tandem Mass Spectrometry Method for Free and Total Mycophenolic Acid

Author(s) -

Frank Streit,

Maria Shipkova,

Victor W. Armstrong,

Michael Oellerich

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.024323

Subject(s) - chromatography , chemistry , high performance liquid chromatography , mass spectrometry , detection limit , tandem mass spectrometry , elution , liquid chromatography–mass spectrometry , selected reaction monitoring , free fraction , biochemistry , plasma protein binding

Because mycophenolic acid (MPA) is highly protein bound and because the free fraction is the pharmacologically active portion, a rapid, reliable, and sensitive procedure is required to study the relationship between free MPA and treatment efficacy/toxicity. Liquid chromatography-tandem mass spectrometry is ideally suited for such a method.

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