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Detection of SARS Coronavirus in Patients with Severe Acute Respiratory Syndrome by Conventional and Real-Time Quantitative Reverse Transcription-PCR Assays
Author(s) -
Leo L. M. Poon,
Kwok Hung Chan,
On Kei Wong,
Timothy Cheung,
Iris H. Y. Ng,
BoJian Zheng,
WH Seto,
KwokYung Yuen,
Yi Guan,
Malik Peiris
Publication year - 2004
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/clinchem.2003.023663
Subject(s) - reverse transcription polymerase chain reaction , covid-19 , real time polymerase chain reaction , virology , reverse transcriptase , coronavirus , medicine , betacoronavirus , respiratory system , sars virus , severe acute respiratory syndrome , biology , polymerase chain reaction , messenger rna , gene , pathology , infectious disease (medical specialty) , disease , outbreak , genetics
A novel coronavirus (CoV) was recently identified as the agent for severe acute respiratory syndrome (SARS). We compared the abilities of conventional and real-time reverse transcription-PCR (RT-PCR) assays to detect SARS CoV in clinical specimens.

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