High-Throughput Mutation Detection Method to Scan BRCA1 and BRCA2 Based on Heteroduplex Analysis by Capillary Array Electrophoresis | Zendy

Eva EstebanCardeñosa | Zendy; M. Durán | Zendy; Mar Infante | Zendy; Eladio A. Velasco | Zendy; Cristina Miner | Zendy

Open Access

High-Throughput Mutation Detection Method to Scan BRCA1 and BRCA2 Based on Heteroduplex Analysis by Capillary Array Electrophoresis

Author(s) -

Eva EstebanCardeñosa,

M. Durán,

Mar Infante,

Eladio A. Velasco,

Cristina Miner

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.023614

Subject(s) - heteroduplex , capillary electrophoresis , microbiology and biotechnology , dna , multiplex , genomic dna , dna sequencer , biology , gel electrophoresis , dna sequencing , genetics , polymerase chain reaction , gene , chemistry

Scanning for mutations in BRCA1 and BRCA2 in a large number of samples is hampered by the large sizes of these genes and the scattering of mutations throughout their coding sequences. Automated capillary electrophoresis has been shown to be a powerful system to detect mutations by either single-strand conformation polymorphism or heteroduplex analysis (HA).

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