The Interleukin-6 G(−174)C Promoter Polymorphism Does Not Determine Plasma Interleukin-6 Concentrations in Experimental Endotoxemia in Humans | Zendy

Georg Endler | Zendy; Claudia Marsik | Zendy; Christian Joukhadar | Zendy; Rodrig Marculescu | Zendy; Florian Mayr | Zendy; Christine Mannhalter | Zendy; Oswald Wagner | Zendy; Bernd Jilma | Zendy

Open Access

The Interleukin-6 G(−174)C Promoter Polymorphism Does Not Determine Plasma Interleukin-6 Concentrations in Experimental Endotoxemia in Humans

Author(s) -

Georg Endler,

Claudia Marsik,

Christian Joukhadar,

Rodrig Marculescu,

Florian Mayr,

Christine Mannhalter,

Oswald Wagner,

Bernd Jilma

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.022459

Subject(s) - genotype , pathogenesis , lipopolysaccharide , cytokine , interleukin , medicine , endocrinology , basal (medicine) , interleukin 6 , immunoassay , polymorphism (computer science) , immunology , biology , gene , antibody , biochemistry , insulin

Background: Interleukin 6 (IL-6) is a pleiotropic cytokine that plays an essential role in the pathogenesis of acute and chronic infections. As the role of the IL-6 G(−174)C polymorphism in determining serum concentrations of IL-6 is controversial, we studied the genotype-specific IL-6 response in a well-standardized model of systemic inflammation. Methods: A total of 76 healthy young males (age range, 19–35 years) received a single bolus of 2 ng/kg endotoxin [lipopolysaccharide (LPS)] intravenously. Plasma IL-6 was measured by enzyme immunoassay at 0, 2, 6, and 24 h after LPS infusion, and the IL-6 promoter genotype was analyzed by a mutagenic separated PCR assay. Results: IL-6 increased 300-fold 2 h after LPS challenge and returned almost to normal within 24 h. Neither basal IL-6 nor the IL-6 response to LPS was significantly affected by the IL-6 promoter genotype. Conclusions: The IL-6 G(−174)C promoter polymorphism does not significantly influence basal concentrations of IL-6 or peak IL-6 in human endotoxemia.

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