Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms | Zendy

Soi-Moi Chye | Zendy; Shiu-Ru Lin | Zendy; YaLei Chen | Zendy; Lee-Yi Chung | Zendy; ChuanMin Yen | Zendy

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Immuno-PCR for Detection of Antigen to Angiostrongylus cantonensis Circulating Fifth-Stage Worms

Author(s) -

Soi-Moi Chye,

Shiu-Ru Lin,

YaLei Chen,

Lee-Yi Chung,

ChuanMin Yen

Publication year - 2004

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/clinchem.2003.020867

Subject(s) - antigen , angiostrongylus cantonensis , biology , antibody , polymerase chain reaction , cerebrospinal fluid , monoclonal antibody , virology , angiostrongyliasis , primer (cosmetics) , immunology , helminths , chemistry , biochemistry , gene , organic chemistry , neuroscience

Definitive diagnosis of infestation with Angiostrongylus cantonensis is difficult because the parasitic nematode is undetectable in the cerebrospinal fluid (CSF) of one-half of afflicted patients and the diagnostic sensitivity of ELISA for circulating worm antigens in patient sera is low. We studied immuno-PCR as a diagnostic tool.

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