Development and Assessment of a Quantitative Reverse Transcription-PCR Assay for Simultaneous Measurement of Four Amplicons | Zendy

Marcy B. Grace | Zendy; Christopher B. McLeland | Zendy; Steven J. Gagliardi | Zendy; Jeffrey M. Smith | Zendy; William E. Jackson | Zendy; William F. Blakely | Zendy

Open Access

Development and Assessment of a Quantitative Reverse Transcription-PCR Assay for Simultaneous Measurement of Four Amplicons

Author(s) -

Marcy B. Grace,

Christopher B. McLeland,

Steven J. Gagliardi,

Jeffrey M. Smith,

William E. Jackson,

William F. Blakely

Publication year - 2003

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/49.9.1467

Subject(s) - amplicon , microbiology and biotechnology , reverse transcriptase , real time polymerase chain reaction , complementary dna , biology , serial dilution , primer (cosmetics) , exonuclease , reverse transcription polymerase chain reaction , rna , gene expression , dna , polymerase chain reaction , chemistry , gene , dna polymerase , biochemistry , medicine , alternative medicine , organic chemistry , pathology

High-throughput and forward-deployable biological dosimetry capabilities are required for tactical and medical decisions after radiologic events. We previously reported a quantitative reverse transcription (QRT)-PCR assay for human radiation-responsive gene targets using a whole-blood ex vivo irradiation model, but we needed a multitarget assay on a smaller, less costly, real-time PCR detection system.

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