Hemoglobin Variants and Hemoglobin A1c Analysis: Problem Solved?

Measurement of glycohemoglobin (GHb) is an integral component of the management of patients with diabetes mellitus (1)(2). Glycation is the nonenzymatic addition of a sugar residue to amino groups of proteins. Numerous proteins in the body are glycated, but GHb in blood is the analyte most widely used clinically to monitor glycemic control. The concentration of GHb is directly proportional to the mean concentration of glucose in the blood and the lifespan of erythrocytes (mean, 120 days). Thus, the GHb concentration represents an integrated value for glucose over the preceding 2–3 months. GHb provides an index of glycemic control that is free of the wide diurnal glucose fluctuations and is unaffected by recent exercise or food ingestion.Two large, prospective, randomized clinical trials demonstrated a strong relationship between hyperglycemia and the development of microvascular complications of diabetes. In the Diabetes Control and Complications Trial (DCCT) and the United Kingdom Prospective Diabetes Study (UKPDS), which studied patients with type 1 and type 2 diabetes, respectively (3)(4), glycemic control was assessed by GHb. Both studies established a direct relationship between the GHb concentration and the risk of complications. These findings led the American Diabetes Association to recommend that a primary treatment goal in adults with diabetes should be near-normal glycemia with hemoglobin A1c (HbA1c) 2 million GHb measurements each month …