High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen | Zendy

Carl T. Wittwer | Zendy; G. H. Reed | Zendy; Cameron N. Gundry | Zendy; Joshua G Vandersteen | Zendy; Robert J. Pryor | Zendy

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High-Resolution Genotyping by Amplicon Melting Analysis Using LCGreen

Author(s) -

Carl T. Wittwer,

G. H. Reed,

Cameron N. Gundry,

Joshua G Vandersteen,

Robert J. Pryor

Publication year - 2003

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/49.6.853

Subject(s) - amplicon , high resolution melt , melting curve analysis , genotyping , microbiology and biotechnology , primer (cosmetics) , biology , polymerase chain reaction , oligonucleotide , genomic dna , genotype , gene , genetics , chemistry , organic chemistry

High-resolution amplicon melting analysis was recently introduced as a closed-tube method for genotyping and mutation scanning (Gundry et al. Clin Chem 2003;49:396-406). The technique required a fluorescently labeled primer and was limited to the detection of mutations residing in the melting domain of the labeled primer. Our aim was to develop a closed-tube system for genotyping and mutation scanning that did not require labeled oligonucleotides.

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