PCR-based Calibration Curves for Studies of Quantitative Gene Expression in Human Monocytes: Development and Evaluation | Zendy

Reidun Øvstebø | Zendy; Kari Bente Foss Haug | Zendy; Knut Lande | Zendy; Peter Kierulf | Zendy

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PCR-based Calibration Curves for Studies of Quantitative Gene Expression in Human Monocytes: Development and Evaluation

Author(s) -

Reidun Øvstebø,

Kari Bente Foss Haug,

Knut Lande,

Peter Kierulf

Publication year - 2003

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/49.3.425

Subject(s) - amplicon , calibration curve , reproducibility , complementary dna , calibration , biology , coefficient of variation , real time polymerase chain reaction , microbiology and biotechnology , reverse transcription polymerase chain reaction , gene expression , standard curve , messenger rna , reverse transcriptase , gene , chemistry , polymerase chain reaction , chromatography , genetics , mathematics , statistics , biochemistry , detection limit

Quantitative reverse transcription-PCR (RT-PCR) used to detect small changes in specific mRNA concentrations is often associated with poor reproducibility. Thus, there is a need for stringent quality control in each step of the protocol.

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