Open AccessAmplicon Melting Analysis with Labeled Primers: A Closed-Tube Method for Differentiating Homozygotes and Heterozygotes
Author(s) -
Cameron N. Gundry,
Joshua G Vandersteen,
G. H. Reed,
Robert J. Pryor,
Jian Chen,
Carl T. Wittwer
Publication year - 2003
Publication title -
clinical chemistry
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.705
H-Index - 218
eISSN - 1530-8561
pISSN - 0009-9147
DOI - 10.1373/49.3.396
Subject(s) - amplicon , heterozygote advantage , tube (container) , chromatography , microbiology and biotechnology , biology , polymerase chain reaction , chemistry , genetics , materials science , genotype , gene , composite material
Common methods for identification of DNA sequence variants use gel electrophoresis or column separation after PCR.
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