HER-2/neu Gene Copy Number Quantified by Real-Time PCR: Comparison of Gene Amplification, Heterozygosity, and Immunohistochemical Status in Breast Cancer Tissue | Zendy

Mélanie Königshoff | Zendy; Jochen Wilhelm | Zendy; Rainer M. Bohle | Zendy; Alfred Pingoud | Zendy; Meinhard Hahn | Zendy

Open Access

HER-2/neu Gene Copy Number Quantified by Real-Time PCR: Comparison of Gene Amplification, Heterozygosity, and Immunohistochemical Status in Breast Cancer Tissue

Author(s) -

Mélanie Königshoff,

Jochen Wilhelm,

Rainer M. Bohle,

Alfred Pingoud,

Meinhard Hahn

Publication year - 2003

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/49.2.219

Subject(s) - loss of heterozygosity , immunohistochemistry , biology , gene duplication , breast cancer , microbiology and biotechnology , real time polymerase chain reaction , cancer , copy number variation , polymerase chain reaction , gene , cancer research , allele , pathology , medicine , genetics , immunology , genome

Amplification of the oncogene HER-2/neu influences breast cancer pathogenesis, and therapy and prognosis may be affected by the degree of amplification. The extent of amplification or protein overexpression typically is analyzed by fluorescence in situ hybridization or immunohistochemistry (IHC), but quantitative PCR techniques have been described that may provide alternatives to these methods.

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