Dual-Label Immunoassay for Simultaneous Measurement of Prostate-specific Antigen (PSA)-α1-Antichymotrypsin Complex Together with Free or Total PSA | Zendy

Lei Zhu | Zendy; Jari Lein | Zendy; Wanming Zhang | Zendy; Patrik Finne | Zendy; UlfHåkan Stenman | Zendy

Open Access

Dual-Label Immunoassay for Simultaneous Measurement of Prostate-specific Antigen (PSA)-α1-Antichymotrypsin Complex Together with Free or Total PSA

Author(s) -

Lei Zhu,

Jari Lein,

Wanming Zhang,

Patrik Finne,

UlfHåkan Stenman

Publication year - 2003

Publication title -

clinical chemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.705

H-Index - 218

eISSN - 1530-8561

pISSN - 0009-9147

DOI - 10.1373/49.1.97

Subject(s) - prostate specific antigen , prostate cancer , chemistry , immunoassay , monoclonal antibody , prostate , antigen , antibody , medicine , cancer , immunology

A major portion of prostate-specific antigen exists in circulation as a complex with alpha(1)-antichymotrypsin (PSA-ACT), whereas a minor part is free (fPSA). The proportion of PSA-ACT is increased in prostate cancer (PCa), but immunologic determination of PSA-ACT is hampered by a background produced by nonspecific adsorption of ACT to the solid phase. To reduce the nonspecific interference, we produced an antibody specific for complexed ACT and developed immunofluorometric assays (IFMAs) for simultaneous measurement of fPSA + PSA-ACT (fPSA/PSA-ACT) and PSA-ACT + total PSA (tPSA, PSA-ACT/tPSA).

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