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Carriage of the genetic combination encoding a high expression inhibitory Killer Immunoglobulin-like Receptor (KIR)3DL1 with its ligand, HLA-B*57 ( *h/*y+B*57 ) is associated with slower time to AIDS and better HIV viral load control than being a  Bw6  homozygote ( Bw6hmz ). Natural Killer (NK) cells from  *h/*y+B*57  carriers receive potent educational signals through HLA-B*57 KIR3DL1 ligation leading to high functional potential. NK cells from  Bw6hmz  are not educated through KIR3DL1 because Bw6 antigens do not interact with this inhibitory receptor. To better understand the impact of KIR/HLA combinations on NK cell mediated anti-viral activity we measured NK cell mediated inhibition of HIV replication in autologous infected CD4 (iCD4) cells by assessing the frequency of p24 positive CD4 targets and supernatant levels of HIV p24 longitudinally in the presence versus absence of NK cells. Forty-seven HIV uninfected subjects were studied, including carriers of  *h/*y+B*57 , a low expression  KIR3DL1  genotype with  HLA-B*57  termed  *l/*x+B*57 , a genotype designated  3DS1+*80I  and  Bw6hmz . NK cells from  *h/*y+B*57  carriers, like those from  3DS1+*80I  subjects, inhibited HIV replication in autologous iCD4 cells better than those from  Bw6hmz  and  *l/*x+B*57  carriers. Cell contact between NK and iCD4 cells activated NK cells to inhibit viral replication in a non-contact dependent fashion through secretion of CC-chemokines. iCD4 stimulated NK cells from  *h/*y+B*57  and  3DS1+*80I  carriers produced higher levels of CC-chemokines than those from  Bw6hmz  or  *l/*x+B*57  carriers. Higher levels of CC-chemokines were produced by KIR3DL1 +  than KIR3DL1 −  NK cells. We conclude that NK-mediated inhibition of viral replication in autologous iCD4 cells is partially due to a block at the level of HIV entry into new targets by secreted CC-chemokines.

HIV Protective KIR3DL1/S1-HLA-B Genotypes Influence NK Cell-Mediated Inhibition of HIV Replication in Autologous CD4 Targets