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Heterologous transinfection with the endosymbiotic bacterium  Wolbachia  has been shown previously to induce pathogen interference phenotypes in mosquito hosts. Here we examine an artificially infected strain of  Aedes polynesiensis , the primary vector of  Wuchereria bancrofti , which is the causative agent of Lymphatic filariasis (LF) throughout much of the South Pacific. Embryonic microinjection was used to transfer the  w AlbB infection from  Aedes albopictus  into an aposymbiotic strain of  Ae. polynesiensis . The resulting strain (designated “MTB”) experiences a stable artificial infection with high maternal inheritance. Reciprocal crosses of MTB with naturally infected wild-type  Ae. polynesiensis  demonstrate strong bidirectional incompatibility. Levels of reactive oxygen species (ROS) in the MTB strain differ significantly relative to that of the wild-type, indicating an impaired ability to regulate oxidative stress. Following a challenge with  Brugia pahangi , the number of filarial worms achieving the infective stage is significantly reduced in MTB as compared to the naturally infected and aposymbiotic strains. Survivorship of MTB differed significantly from that of the wild-type, with an interactive effect between survivorship and blood feeding. The results demonstrate a direct correlation between decreased ROS levels and decreased survival of adult female  Aedes polynesiensis . The results are discussed in relation to the interaction of  Wolbachia  with ROS production and antioxidant expression, iron homeostasis and the insect immune system. We discuss the potential applied use of the MTB strain for impacting  Ae. polynesiensis  populations and strategies for reducing LF incidence in the South Pacific.

Reactive Oxygen Species Production and Brugia pahangi Survivorship in Aedes polynesiensis with Artificial Wolbachia Infection Types