Open AccessDissecting the Cell Entry Pathway of Dengue Virus by Single-Particle Tracking in Living Cells
Author(s) -
Hilde M. van der Schaar,
Michael J. Rust,
Chen Chen,
Heidi van der Ende-Metselaar,
Jan Wilschut,
Xiaowei Zhuang,
Jolanda M. Smit
Publication year - 2008
Publication title -
plos pathogens
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.719
H-Index - 206
eISSN - 1553-7374
pISSN - 1553-7366
DOI - 10.1371/journal.ppat.1000244
Subject(s) - endosome , endocytic cycle , endocytosis , dengue virus , microbiology and biotechnology , lipid bilayer fusion , clathrin , viral entry , biology , virology , virus , cell , viral envelope , flavivirus , viral replication , intracellular , genetics
Dengue virus (DENV) is an enveloped RNA virus that causes the most common arthropod-borne infection worldwide. The mechanism by which DENV infects the host cell remains unclear. In this work, we used live-cell imaging and single-virus tracking to investigate the cell entry, endocytic trafficking, and fusion behavior of DENV. Simultaneous tracking of DENV particles and various endocytic markers revealed that DENV enters cells exclusively via clathrin-mediated endocytosis. The virus particles move along the cell surface in a diffusive manner before being captured by a pre-existing clathrin-coated pit. Upon clathrin-mediated entry, DENV particles are transported to Rab5-positive endosomes, which subsequently mature into late endosomes through acquisition of Rab7 and loss of Rab5. Fusion of the viral membrane with the endosomal membrane was primarily detected in late endosomal compartments.
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