Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme | Zendy

Linda Grillová | Zendy; Tanika Bawa | Zendy; Lenka Mikalová | Zendy; Angèle GayetAgeron | Zendy; Kay Nieselt | Zendy; Michal Strouhal | Zendy; Patrice Sednaoui | Zendy; Tristan Ferry | Zendy; Matthias Cavassini | Zendy; Stephan Lautenschlager | Zendy; Fabrizio Dutly | Zendy; Marta Pla-Díaz | Zendy; Michael Krützen | Zendy; Fernando GónzálezCandelas | Zendy; Homayoun C. Bagheri | Zendy; David Šmajs | Zendy; Natasha Arora | Zendy; Philipp P. Bosshard | Zendy

Open Access

Molecular characterization of Treponema pallidum subsp. pallidum in Switzerland and France with a new multilocus sequence typing scheme

Author(s) -

Linda Grillová,

Tanika Bawa,

Lenka Mikalová,

Angèle GayetAgeron,

Kay Nieselt,

Michal Strouhal,

Patrice Sednaoui,

Tristan Ferry,

Matthias Cavassini,

Stephan Lautenschlager,

Fabrizio Dutly,

Marta Pla-Díaz,

Michael Krützen,

Fernando GónzálezCandelas,

Homayoun C. Bagheri,

David Šmajs,

Natasha Arora,

Philipp P. Bosshard

Publication year - 2018

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0200773

Subject(s) - multilocus sequence typing , biology , typing , treponema , genetics , clade , gene , virology , phylogenetics , genotype , syphilis , human immunodeficiency virus (hiv)

Syphilis is an important public health problem and an increasing incidence has been noted in recent years. Characterization of strain diversity through molecular data plays a critical role in the epidemiological understanding of this re-emergence. We here propose a new high-resolution multilocus sequence typing (MLST) scheme for Treponema pallidum subsp. pallidum (TPA). We analyzed 30 complete and draft TPA genomes obtained directly from clinical samples or from rabbit propagated strains to identify suitable typing loci and tested the new scheme on 120 clinical samples collected in Switzerland and France. Our analyses yielded three loci with high discriminatory power: TP0136, TP0548, and TP0705. Together with analysis of the 23S rRNA gene mutations for macrolide resistance, we propose these loci as MLST for TPA. Among clinical samples, 23 allelic profiles as well as a high percentage (80% samples) of macrolide resistance were revealed. The new MLST has higher discriminatory power compared to previous typing schemes, enabling distinction of TPA from other treponemal bacteria, distinction between the two main TPA clades (Nichols and SS14), and differentiation of strains within these clades.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research