Open AccessThe Dox-pDC - A murine conditionally immortalized plasmacytoid dendritic cell line with native immune profile
Author(s) -
Sebastian Thieme,
Alexander Holzbaur,
Ralf Wiedemuth,
Aline Binner,
Katrin Navratiel,
Konstantinos Anastassiadis,
Sebastian Brenner,
Cornelia Richter
Publication year - 2018
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0192437
Subject(s) - plasmacytoid dendritic cell , immune system , microbiology and biotechnology , population , biology , dendritic cell , ex vivo , immunology , antigen presentation , t cell , cell culture , antigen , antigen presenting cell , immortalised cell line , in vivo , medicine , genetics , environmental health
Plasmacytoid dendritic cells (pDC) constitute a very rare blood cell population and play a significant role in immune response and immune-mediated disorders. Investigations on primary pDCs are hindered not only due to their rarity but also because they represent a heterogeneous cell population which is difficult to culture ex vivo . We generated a conditionally immortalized pDC line (Dox-pDC) from mice with Doxycycline-inducible SV40 Large T Antigen with a comparable immune profile to primary pDCs. The Dox-pDC secrete pro- and anti-inflammatory cytokines upon Toll-like receptor 9 stimulation and upregulate their MHCI, MHCII and costimulatory molecules. Further, the Dox-pDC activate and polarize naïve T cells in vivo and in vitro in response to the model antigen Ovalbumin. Due to their long-term culture stability and their robust proliferation Dox-pDC represent a reliable alternative to primary mouse pDC.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom