Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum | Zendy

Bartłomiej Dudek | Zendy; Eva Krzyżewska | Zendy; Katarzyna Kapczyńska | Zendy; Jacek Rybka | Zendy; Aleksandra Pawlak | Zendy; Kamila Korzekwa | Zendy; Elżbieta Klausa | Zendy; Gabriela BuglaPłoskońska | Zendy

Open Access

Proteomic Analysis of Outer Membrane Proteins from Salmonella Enteritidis Strains with Different Sensitivity to Human Serum

Author(s) -

Bartłomiej Dudek,

Eva Krzyżewska,

Katarzyna Kapczyńska,

Jacek Rybka,

Aleksandra Pawlak,

Kamila Korzekwa,

Elżbieta Klausa,

Gabriela BuglaPłoskońska

Publication year - 2016

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0164069

Subject(s) - bacterial outer membrane , salmonella enteritidis , microbiology and biotechnology , lipopolysaccharide , membrane protein , biology , bacteria , chemistry , salmonella , membrane , biochemistry , escherichia coli , gene , genetics , endocrinology

Differential analysis of outer membrane composition of S . Enteritidis strains, resistant to 50% normal human serum (NHS) was performed in order to find factors influencing the resistance to higher concentrations of NHS. Ten S . Enteritidis clinical strains, resistant to 50% NHS, all producing very long lipopolysaccharide, were subjected to the challenge of 75% NHS. Five extreme strains: two resistant and three sensitive to 75% NHS, were chosen for the further analysis of outer membrane proteins composition. Substantial differences were found in the levels of particular outer membrane proteins between resistant and sensitive strains, i.e. outer membrane protease E (PgtE) was present mainly in resistant strains, while sensitive strains possessed a high level of flagellar hook-associated protein 2 (FliD) and significantly higher levels of outer membrane protein A (OmpA).

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