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In the central nervous system (CNS) platelet derived growth factor receptor alpha (PDGFRα) is expressed exclusively by oligodendrocyte progenitor cells (OPCs), making the  Pdgfrα  promoter an ideal tool for directing transgene expression in this cell type. Two  Pdgfrα-CreER   T2   mouse lines have been generated for this purpose which, when crossed with cre-sensitive reporter mice, allow the temporally restricted labelling of OPCs for lineage-tracing studies. These mice have also been used to achieve the deletion of CNS-specific genes from OPCs. However the ability of  Pdgfrα-CreER   T2   mice to induce cre-mediated recombination in PDGFR α  +  cell populations located outside of the CNS has not been examined. Herein we quantify the proportion of PDGFRα +  cells that become YFP-labelled following Tamoxifen administration to adult  Pdgfrα-CreER   T2  :: Rosa26-YFP  transgenic mice. We report that the vast majority (>90%) of PDGFRα +  OPCs in the CNS, and a significant proportion of PDGFRα +  stromal cells within the bone marrow (~38%) undergo recombination and become YFP-labelled. However, only a small proportion of the PDGFRα +  cell populations found in the sciatic nerve, adrenal gland, pituitary gland, heart, gastrocnemius muscle, kidney, lung, liver or intestine become YFP-labelled. These data suggest that  Pdgfrα-CreER   T2   transgenic mice can be used to achieve robust recombination in OPCs, while having a minimal effect on most PDGFRα +  cell populations outside of the CNS.

Evaluating Tissue-Specific Recombination in a Pdgfrα-CreERT2 Transgenic Mouse Line