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The maintenance of potassium homeostasis is crucial for all types of cells, including  Candida glabrata . Three types of plasma-membrane systems mediating potassium influx with different transport mechanisms have been described in yeasts: the Trk1 uniporter, the Hak cation-proton symporter and the Acu ATPase. The  C .  glabrata  genome contains only one gene encoding putative system for potassium uptake, the Trk1 uniporter. Therefore, its importance in maintaining adequate levels of intracellular potassium appears to be critical for  C .  glabrata  cells. In this study, we first confirmed the potassium-uptake activity of the identified gene’s product by heterologous expression in a suitable  S .  cerevisiae  mutant, further we generated a corresponding deletion mutant in  C .  glabrata  and analysed its phenotype in detail. The obtained results show a pleiotropic effect on the cell physiology when  CgTRK1  is deleted, affecting not only the ability of  trk1Δ  to grow at low potassium concentrations, but also the tolerance to toxic alkali-metal cations and cationic drugs, as well as the membrane potential and intracellular pH. Taken together, our results find the sole potassium uptake system in  C .  glabrata  cells to be a promising target in the search for its specific inhibitors and in developing new antifungal drugs.

Potassium Uptake Mediated by Trk1 Is Crucial for Candida glabrata Growth and Fitness