A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections | Zendy

Scott T. Moen | Zendy; Christopher L. Hatcher | Zendy; Anup K. Singh | Zendy

Open Access

A Centrifugal Microfluidic Platform That Separates Whole Blood Samples into Multiple Removable Fractions Due to Several Discrete but Continuous Density Gradient Sections

Author(s) -

Scott T. Moen,

Christopher L. Hatcher,

Anup K. Singh

Publication year - 2016

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0153137

Subject(s) - microfluidics , density gradient , visualization , volume (thermodynamics) , centrifugation , sample (material) , chromatography , biomedical engineering , differential centrifugation , biological system , materials science , computer science , chemistry , biology , nanotechnology , microbiology and biotechnology , data mining , physics , medicine , quantum mechanics

We present a miniaturized centrifugal platform that uses density centrifugation for separation and analysis of biological components in small volume samples (~5 μL). We demonstrate the ability to enrich leukocytes for on-disk visualization via microscopy, as well as recovery of viable cells from each of the gradient partitions. In addition, we simplified the traditional Modified Wright-Giemsa staining by decreasing the time, volume, and expertise involved in the procedure. From a whole blood sample, we were able to extract 95.15% of leukocytes while excluding 99.8% of red blood cells. This platform has great potential in both medical diagnostics and research applications as it offers a simpler, automated, and inexpensive method for biological sample separation, analysis, and downstream culturing.

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