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Calcineurin is a calcium/calmodulin dependent protein phosphatase in eukaryotes that consists of a catalytic subunit A and a regulatory subunit B. Previous studies in the filamentous fungus  Neurospora crassa  had suggested that the catalytic subunit of calcineurin might be an essential protein. We generated  N .  crassa  strains expressing the A ( cna-1 ) and B ( cnb-1 ) subunit genes under the regulation of  P   tcu-1  , a copper-responsive promoter. In these strains, addition of bathocuproinedisulfonic acid (BCS), a copper chelator, results in induction of  cna-1  and  cnb-1 , while excess Cu 2+  represses gene expression. Through analysis of these strains under repressing and inducing conditions, we found that the calcineurin is required for normal growth, asexual development and female fertility in  N .  crassa . Moreover, we isolated and analyzed  cnb-1  mutant alleles generated by repeat-induced point mutation (RIP), with the results further supporting roles for calcineurin in growth and fertility in  N .  crassa . We demonstrated a direct interaction between the CNA-1 and CNB-1 proteins using an assay system developed to study protein-protein interactions in  N .  crassa .

Calcineurin Subunits A and B Interact to Regulate Growth and Asexual and Sexual Development in Neurospora crassa