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Fishes are one of the most intensively studied marine taxonomic groups yet cryptic species are still being discovered. An integrated taxonomic approach is used herein to delineate and describe a new cryptic seamoth (genus  Pegasus ) from what was previously a wide-ranging species. Preliminary mitochondrial DNA barcoding indicated possible speciation in  Pegasus volitans  specimens collected in surveys of the Torres Strait and Great Barrier Reef off Queensland in Australia. Morphological and meristic investigations found key differences in a number of characters between  P .  volitans  and the new species,  P .  tetrabelos . Further mt DNA barcoding of both the  COI  and the slower mutating  16S  genes of additional specimens provided strong support for two separate species.  Pegasus tetrabelos  and  P .  volitans  are sympatric in northern Australia and were frequently caught together in trawls at the same depths.

Integrated Taxonomy Reveals Hidden Diversity in Northern Australian Fishes: A New Species of Seamoth (Genus Pegasus)