Transcriptome Analysis of an Anthracnose-Resistant Tea Plant Cultivar Reveals Genes Associated with Resistance to Colletotrichum camelliae | Zendy

Lu Wang | Zendy; Yuchun Wang | Zendy; Hongli Cao | Zendy; Xinyuan Hao | Zendy; Jianming Zeng | Zendy; Yajun Yang | Zendy; Xinchao Wang | Zendy

Open Access

Transcriptome Analysis of an Anthracnose-Resistant Tea Plant Cultivar Reveals Genes Associated with Resistance to Colletotrichum camelliae

Author(s) -

Lu Wang,

Yuchun Wang,

Hongli Cao,

Xinyuan Hao,

Jianming Zeng,

Yajun Yang,

Xinchao Wang

Publication year - 2016

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0148535

Subject(s) - phenylpropanoid , biology , transcriptome , secondary metabolism , wrky protein domain , colletotrichum , plant disease resistance , gene , phenylalanine ammonia lyase , camellia sinensis , flavonoid biosynthesis , metabolic pathway , plant hormone , antibiosis , botany , gene expression , biosynthesis , genetics , phenylalanine , bacteria , amino acid

Tea plant breeding is a topic of great economic importance. However, disease remains a major cause of yield and quality losses. In this study, an anthracnose-resistant cultivar, ZC108, was developed. An infection assay revealed different responses to Colletotrichum sp. infection between ZC108 and its parent cultivar LJ43. ZC108 had greater resistance than LJ43 to Colletotrichum camelliae . Additionally, ZC108 exhibited earlier sprouting in the spring, as well as different leaf shape and plant architecture. Microarray data revealed that the genes that are differentially expressed between LJ43 and ZC108 mapped to secondary metabolism-related pathways, including phenylpropanoid biosynthesis, phenylalanine metabolism, and flavonoid biosynthesis pathways. In addition, genes involved in plant hormone biosynthesis and signaling as well as plant-pathogen interaction pathways were also changed. Quantitative real-time PCR was used to examine the expression of 27 selected genes in infected and uninfected tea plant leaves. Genes encoding a MADS-box transcription factor, NBS-LRR disease-resistance protein, and phenylpropanoid metabolism pathway components (CAD, CCR, POD, beta-glucosidase, ALDH and PAL) were among those differentially expressed in ZC108.

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