MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes | Zendy

Linyuan Shen | Zendy; Lei Chen | Zendy; Shunhua Zhang | Zendy; Jingjing Du | Zendy; Lin Bai | Zendy; Yi Zhang | Zendy; Yanzhi Jiang | Zendy; Xuewei Li | Zendy; Jinyong Wang | Zendy; Li Zhu | Zendy

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MicroRNA-27b Regulates Mitochondria Biogenesis in Myocytes

Author(s) -

Linyuan Shen,

Lei Chen,

Shunhua Zhang,

Jingjing Du,

Lin Bai,

Yi Zhang,

Yanzhi Jiang,

Xuewei Li,

Jinyong Wang,

Li Zhu

Publication year - 2016

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0148532

Subject(s) - c2c12 , mitochondrial biogenesis , microrna , microbiology and biotechnology , biology , nrf1 , mitochondrion , biogenesis , mef2c , three prime untranslated region , regulation of gene expression , myocyte , organelle biogenesis , tfam , gene expression , untranslated region , gene , messenger rna , genetics , myogenesis

MicroRNAs (miRNAs) are small, non-coding RNAs that affect the post-transcriptional regulation of various biological pathways. To date, it is not fully understood how miRNAs regulate mitochondrial biogenesis. This study aimed at the identification of the role of miRNA-27b in mitochondria biogenesis. The mitochondria content in C2C12 cells was significantly increased during myogenic differentiation and accompanied by a marked decrease of miRNA-27b expression. Furthermore, the expression of the predicted target gene of miRNA-27b, forkhead box j3 ( Foxj3 ), was also increased during myogenic differentiation. Luciferase activity assays confirmed that miRNA-27b directly targets the 3’-untranslated region (3’-UTR) of Foxj3 . Overexpression of miRNA-27b provoked a decrease of mitochondria content and diminished expression of related mitochondrial genes and Foxj3 both at mRNA and protein levels. The expression levels of downstream genes of Foxj3 , such as Mef2c , PGC1α , NRF1 and mtTFA , were also decreased in C2C12 cells upon overexpression of miRNA-27b. These results suggested that miRNA-27b may affect mitochondria biogenesis by down-regulation of Foxj3 during myocyte differentiation.

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