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Different engineered organisms have been used to produce L-lactate. Poor yields of lactate at low pH and expensive downstream processing remain as bottlenecks.  Aspergillus niger  is a prolific citrate producer and a remarkably acid tolerant fungus. Neither a functional lactate dehydrogenase (LDH) from nor lactate production by  A .  niger  is reported. Its genome was also investigated for the presence of a functional  ldh . The endogenous  A .  niger  citrate synthase promoter relevant to  A .  niger  acidogenic metabolism was employed to drive constitutive expression of mouse lactate dehydrogenase ( mldhA ). An appraisal of different branches of the  A .  niger  pyruvate node guided the choice of  mldhA  for heterologous expression. A high copy number transformant C12 strain, displaying highest LDH specific activity, was analyzed under different growth conditions. The C12 strain produced 7.7 g/l of extracellular L-lactate from 60 g/l of glucose, in non-neutralizing minimal media. Significantly, lactate and citrate accumulated under two different growth conditions. Already an established acidogenic platform,  A .  niger  now promises to be a valuable host for lactate production.

Expression of Lactate Dehydrogenase in Aspergillus niger for L-Lactic Acid Production