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Background    Seadornavirus  is a genus of viruses in the family  Reoviridae , which consists of  Banna virus ,  Kadipiro virus , and  Liao ning virus .  Banna virus  is considered a potential pathogen for zoonotic diseases. Here, we describe a newly discovered  Seadornavirus  isolated from mosquitos ( Culex tritaeniorhynchus ) in Yunnan Province, China, which is related to  Banna virus , and referred to as  Mangshi virus .    Methods and Results  The  Mangshi virus  was isolated by cell culture in  Aedes albopictus  C6/36 cells, in which it replicated and caused cytopathic effects, but not in mammalian BHK-21 or Vero cells. Polyacrylamide gel analysis revealed a genome consisting of 12 segments of double-stranded RNA, with a “6–4–2” pattern in which the migrating bands were different from those of the  Banna virus . Complete genome sequencing was performed by full-length amplification of cDNAs. Sequence analysis showed that seven highly conserved nucleotides and three highly conserved nucleotides were present at the ends of the 5′- and 3′-UTRs in each of 12 genome segments. The amino acid identities of  Mangshi virus  shared with  Balaton virus  varied from 27.3% (VP11) to 72.3% (VP1) with  Banna virus  varying from 18.0% (VP11) to 63.9% (VP1). Phylogenetic analysis based on amino acid sequences demonstrated that  Mangshi virus  is a member of the genus  Seadornavirus  and is most closely related to, but distinct from,  Balaton virus  and  Banna virus  in the genus  Seadornavirus  of the family  Reoviridae .    Conclusion    Mangshi virus  isolated from mosquitoes ( C .  tritaeniorhynchus ) was identified as a newly discovered virus in the genus  Seadornavirus  and is phylogenetically close to  Banna virus , suggesting that there is genetic diversity of seadornaviruses in tropical and subtropical areas of Southeast Asia.

Isolation and Genetic Characterization of Mangshi Virus: A Newly Discovered Seadornavirus of the Reoviridae Family Found in Yunnan Province, China