PFTK1 Promotes Gastric Cancer Progression by Regulating Proliferation, Migration and Invasion | Zendy

Lei Yang | Zendy; Jia Zhu | Zendy; Hua Huang | Zendy; Qichang Yang | Zendy; Jing Cai | Zendy; Qiuhong Wang | Zendy; Junya Zhu | Zendy; Mengting Shao | Zendy; Jinzhang Xiao | Zendy; Jie Cao | Zendy; Xiaodan Gu | Zendy; Shusen Zhang | Zendy; Yingying Wang | Zendy

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PFTK1 Promotes Gastric Cancer Progression by Regulating Proliferation, Migration and Invasion

Author(s) -

Lei Yang,

Jia Zhu,

Hua Huang,

Qichang Yang,

Jing Cai,

Qiuhong Wang,

Junya Zhu,

Mengting Shao,

Jinzhang Xiao,

Jie Cao,

Xiaodan Gu,

Shusen Zhang,

Yingying Wang

Publication year - 2015

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0140451

Subject(s) - cancer , cancer research , biology , cell growth , cell cycle , cell migration , cancer cell , immunohistochemistry , tumor progression , gene knockdown , pathology , medicine , cell , immunology , cell culture , genetics

PFTK1, also known as PFTAIRE1, CDK14, is a novel member of Cdc2-related serine/threonine protein kinases. Recent studies show that PFTK1 is highly expressed in several malignant tumors such as hepatocellular carcinoma, esophageal cancer, breast cancer, and involved in regulation of cell cycle, tumors proliferation, migration, and invasion that further influence the prognosis of tumors. However, the expression and physiological significance of PFTK1 in gastric cancer remain unclear. In this study, we analyzed the expression and clinical significance of PFTK1 by Western blot in 8 paired fresh gastric cancer tissues, nontumorous gastric mucosal tissues and immunohistochemistry on 161 paraffinembedded slices. High PFTK1 expression was correlated with the tumor grade, lymph node invasion as well as Ki-67. Through Cell Counting Kit (CCK)-8 assay, flow cytometry, colony formation, wound healing and transwell assays, the vitro studies demonstrated that PFTK1 overexpression promoted proliferation, migration and invasion of gastric cancer cells, while PFTK1 knockdown led to the opposite results. Our findings for the first time supported that PFTK1 might play an important role in the regulation of gastric cancer proliferation, migration and would provide a novel promising therapeutic strategy against human gastric cancer.

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