Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams | Zendy

Ana Patrícia C. Silva | Zendy; Auricélio A. Macêdo | Zendy; Luciana Fachini da Costa | Zendy; Cláudia Emanuela Rocha | Zendy; Luize Néli Nunes Garcia | Zendy; Jade Raquel Dias Farias | Zendy; Priscilla Parreira Rocha Gomes | Zendy; Gustavo C. Teixeira | Zendy; Kessler W. J. Fonseca | Zendy; Andréa R. F. Maia | Zendy; Gabriela G. Neves | Zendy; Everton L. Romão | Zendy; Teane M. A. Silva | Zendy; Juliana Pinto da Silva Mol | Zendy; Renata Maria de Oliveira | Zendy; Márcio Sobreira Silva Araújo | Zendy; Ernane F. Nascimento | Zendy; Olindo Assis MartinsFilho | Zendy; Humberto de Mello Brandão | Zendy; Tatiane A. Paíxão | Zendy; Renato L. Santos | Zendy

Open Access

Encapsulated Brucella ovis Lacking a Putative ATP-Binding Cassette Transporter (ΔabcBA) Protects against Wild Type Brucella ovis in Rams

Author(s) -

Ana Patrícia C. Silva,

Auricélio A. Macêdo,

Luciana Fachini da Costa,

Cláudia Emanuela Rocha,

Luize Néli Nunes Garcia,

Jade Raquel Dias Farias,

Priscilla Parreira Rocha Gomes,

Gustavo C. Teixeira,

Kessler W. J. Fonseca,

Andréa R. F. Maia,

Gabriela G. Neves,

Everton L. Romão,

Teane M. A. Silva,

Juliana Pinto da Silva Mol,

Renata Maria de Oliveira,

Márcio Sobreira Silva Araújo,

Ernane F. Nascimento,

Olindo Assis MartinsFilho,

Humberto de Mello Brandão,

Tatiane A. Paíxão,

Renato L. Santos

Publication year - 2015

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0136865

Subject(s) - ovis , biology , brucellosis , brucella , semen , virology , microbiology and biotechnology , brucellaceae , ovis canadensis , brucella melitensis , medicine , genetics , ecology , population , environmental health

This study aimed to evaluate protection induced by the vaccine candidate B . ovis ΔabcBA against experimental challenge with wild type B . ovis in rams. Rams were subcutaneously immunized with B . ovis Δ abcBA encapsulated with sterile alginate or with the non encapsulated vaccine strain. Serum, urine, and semen samples were collected during two months after immunization. The rams were then challenged with wild type B . ovis (ATCC25840), and the results were compared to non immunized and experimentally challenged rams. Immunization, particularly with encapsulated B . ovis Δ abcBA , prevented infection, secretion of wild type B . ovis in the semen and urine, shedding of neutrophils in the semen, and the development of clinical changes, gross and microscopic lesions induced by the wild type B . ovis reference strain. Collectively, our data indicates that the B . ovis Δ abcBA strain is an exceptionally good vaccine strain for preventing brucellosis caused by B . ovis infection in rams.

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