Open AccessThe Fecal Virome of Children with Hand, Foot, and Mouth Disease that Tested PCR Negative for Pathogenic Enteroviruses
Author(s) -
Piyada Linsuwa,
Yong Poovorawan,
Linlin Li,
Xutao Deng,
Sompong Vongpunsawad,
Eric Delwart
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0135573
Subject(s) - human virome , virology , coxsackievirus , enterovirus , biology , echovirus , caliciviridae , picornaviridae , feces , rhinovirus , astrovirus , picornavirus , reoviridae , virus , outbreak , aphthovirus , viral disease , foot and mouth disease , rotavirus , microbiology and biotechnology , metagenomics , genetics , rna , gene
Hand, foot, and mouth disease (HFMD) affects infant and young children. A viral metagenomic approach was used to identify the eukaryotic viruses in fecal samples from 29 Thai children with clinical diagnosis of HFMD collected during the 2012 outbreak. These children had previously tested negative by PCR for enterovirus 71 and coxsackievirus A16 and A6. Deep sequencing revealed nine virus families: Picornaviridae , Astroviridae , Parvoviridae , Caliciviridae , Paramyxoviridae , Adenoviridae , Reoviridae , Picobirnaviridae , and Polyomaviridae . The highest number of viral sequences belonged to human rhinovirus C, astrovirus-MLB2, and coxsackievirus A21. Our study provides an overview of virus community and highlights a broad diversity of viruses found in feces from children with HFMD.
Accelerating Research
Robert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom
Address
John Eccles HouseRobert Robinson Avenue,
Oxford Science Park, Oxford
OX4 4GP, United Kingdom