Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy | Zendy

Cong Zhang | Zendy; Samuel Bélanger | Zendy; Philippe Pouliot | Zendy; Frédéric Lesage | Zendy

Open Access

Measurement of Local Partial Pressure of Oxygen in the Brain Tissue under Normoxia and Epilepsy with Phosphorescence Lifetime Microscopy

Author(s) -

Cong Zhang,

Samuel Bélanger,

Philippe Pouliot,

Frédéric Lesage

Publication year - 2015

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0135536

Subject(s) - phosphorescence , partial pressure , oxygen , microscopy , epilepsy , materials science , confocal microscopy , oxygen pressure , nuclear magnetic resonance , confocal , biomedical engineering , chemistry , optics , neuroscience , medicine , biology , pathology , fluorescence , physics , organic chemistry

In this work a method for measuring brain oxygen partial pressure with confocal phosphorescence lifetime microscopy system is reported. When used in conjunction with a dendritic phosphorescent probe, Oxyphor G4, this system enabled minimally invasive measurements of oxygen partial pressure (pO2) in cerebral tissue with high spatial and temporal resolution during 4-AP induced epileptic seizures. Investigating epileptic events, we characterized the spatio-temporal distribution of the "initial dip" in pO2 near the probe injection site and along nearby arterioles. Our results reveal a correlation between the percent change in the pO2 signal during the "initial dip" and the duration of seizure-like activity, which can help localize the epileptic focus and predict the length of seizure.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research