English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Salmonella enterica  is a zoonotic foodborne pathogen that causes acute gastroenteritis in humans. We assessed the virulence potential of one-hundred and six  Salmonella  strains isolated from food animals and products. A high through-put virulence genes microarray demonstrated  Salmonella  Pathogenicity Islands (SPI) and adherence genes were highly conserved, while prophages and virulence plasmid genes were variably present. Isolates were grouped by serotype, and virulence plasmids separated  S . Typhimurium in two clusters. Atypical microarray results lead to whole genome sequencing (WGS) of  S . Infantis Sal147, which identified deletion of thirty-eight SPI-1 genes. Sal147 was unable to invade HeLa cells and showed reduced mortality in  Galleria mellonella  infection model, in comparison to a SPI-1 harbouring  S . Infantis. Microarray and WGS of  S . Typhimurium Sal199, established for the first time in  S . Typhimurium presence of  cdtB  and other Typhi-related genes. Characterization of Sal199 showed  cdtB  genes were upstream of transposase  IS 911, and co-expressed with other Typhi-related genes. Cell cycle arrest, cytoplasmic distension, and nuclear enlargement were detected in HeLa cells infected by Sal199, but not with  S . Typhimurium LT2. Increased mortality of  Galleria  was detected on infection with Sal199 compared to LT2. Thus,  Salmonella  isolates were rapidly characterized using a high through-put microarray; helping to identify unusual virulence features which were corroborated by further characterisation. This work demonstrates that the use of suitable screening methods for  Salmonella  virulence can help assess the potential risk associated with certain  Salmonella  to humans. Incorporation of such methodology into surveillance could help reduce the risk of emergence of epidemic  Salmonella  strains.

plos one

Virulence Characterization of Salmonella enterica by a New Microarray: Detection and Evaluation of the Cytolethal Distending Toxin Gene Activity in the Unusual Host S. Typhimurium