Open AccessPre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
Author(s) -
Elenice Souza dos Reis Goes,
J. A. F. de Lara,
Eliane Gasparino,
Ana Paula Del Vesco,
Márcio Douglas Goes,
Luiz Alexandre Filho,
Ricardo Pereira Ribeiro
Publication year - 2015
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0129145
Subject(s) - nile tilapia , ryanodine receptor , ryr1 , water holding capacity , receptor , tilapia , gene expression , chemistry , biology , food science , fish <actinopterygii> , fishery , zoology , gene , oreochromis , biochemistry
Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets.
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