The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods | Zendy

Kim van der Zwaluw | Zendy; Angela de Haan | Zendy; Gerlinde Pluister | Zendy; Hester J. Bootsma | Zendy; Albert J. de Neeling | Zendy; Leo M. Schouls | Zendy

Open Access

The Carbapenem Inactivation Method (CIM), a Simple and Low-Cost Alternative for the Carba NP Test to Assess Phenotypic Carbapenemase Activity in Gram-Negative Rods

Author(s) -

Kim van der Zwaluw,

Angela de Haan,

Gerlinde Pluister,

Hester J. Bootsma,

Albert J. de Neeling,

Leo M. Schouls

Publication year - 2015

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0123690

Subject(s) - acinetobacter baumannii , enterobacter cloacae , klebsiella pneumoniae , pseudomonas aeruginosa , carbapenem , biology , enterobacteriaceae , gram , microbiology and biotechnology , enterobacter , escherichia coli , phenotype , cephalosporin , acinetobacter , gene , antibiotics , bacteria , genetics

A new phenotypic test, called the Carbapenem Inactivation Method (CIM), was developed to detect carbapenemase activity in Gram-negative rods within eight hours. This method showed high concordance with results obtained by PCR to detect genes coding for the carbapenemases KPC, NDM, OXA-48, VIM, IMP and OXA-23. It allows reliable detection of carbapenemase activity encoded by various genes in species of Enterobacteriaceae (e.g., Klebsiella pneumoniae , Escherichia coli and Enterobacter cloacae ), but also in non-fermenters Pseudomonas aeruginosa and Acinetobacter baumannii . The CIM was shown to be a cost-effective and highly robust phenotypic screening method that can reliably detect carbapenemase activity.

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