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Glutathione S-transferases (GSTs) compose a family of multifunctional enzymes that play important roles in the detoxification of xenobiotics and the oxidative stress response. In the present study, twenty four GST genes from the transcriptome of a metal-tolerant dark septate endophyte (DSE),  Exophiala pisciphila , were identified based on sequence homology, and their responses to various heavy metal exposures were also analyzed. Phylogenetic analysis showed that the 24 GST genes from  E .  pisciphila  ( EpGST s) were divided into eight distinct classes, including seven cytosolic classes and one mitochondrial metaxin 1-like class. Moreover, the variable expression patterns of these  EpGST s were observed under different heavy metal stresses at their effective concentrations for inhibiting growth by 50% (EC 50 ). Lead (Pb) exposure caused the up-regulation of all  EpGST s, while cadmium (Cd), copper (Cu) and zinc (Zn) treatments led to the significant up-regulation of most of the  EpGST s ( p  < 0.05 to  p  < 0.001). Furthermore, although heavy metal-specific differences in performance were observed under various heavy metals in  Escherichia coli  BL21 (DE3) transformed with  EpGSTN-31 , the over-expression of this gene was able to enhance the heavy metal tolerance of the host cells. These results indicate that  E .  Pisciphila  harbored a diverse of GST genes and the up-regulated  EpGST s are closely related to the heavy metal tolerance of  E .  pisciphila . The study represents the first investigation of the GST family in  E .  pisciphila  and provides a primary interpretation of heavy metal detoxification for  E .  pisciphila .

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Identification of Glutathione S-Transferase (GST) Genes from a Dark Septate Endophytic Fungus (Exophiala pisciphila) and Their Expression Patterns under Varied Metals Stress