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Riemerella anatipestifer  is one of the most important bacterial pathogen of ducks and causes a contagious septicemia.  R .  anatipestifer  infection causes serositis syndromes similar to other bacterial infections in ducks, including infection by  Escherichia coli ,  Salmonella enterica  and  Pasteurella multocida . Clinically differentiating  R .  anatipestifer  infections from other bacterial pathogen infections is usually difficult. In this study, MAb 1G2F10, a monoclonal antibody against  R .  anatipestifer  GroEL, was used to develop a colloidal gold immunochromatographic strip. Colloidal gold particles were prepared by chemical synthesis to an average diameter of 20±5.26 nm by transmission electron microscope imaging. MAb 1G2F10 was conjugated to colloidal gold particles and the formation of antibody-colloidal gold conjugates was monitored by UV/Vis spectroscopy. Immunochromatographic strips were assembled in regular sequence through different accessories sticked on PVC plate. Strips specifically detected  R .  anatipestifer  within 10 min, but did not detect  E .  coli ,  S .  enterica  and  P .  multocida . The detection limit for  R .  anatipestifer  was 1×10 6  colony forming units, which was 500 times higher than a conventional agglutination test. Accuracy was 100% match to multiplex PCR. Assay stability and reproducibility were excellent after storage at 4°C for 6 months. The immunochromatographic strips prepared in this study offer a specific, sensitive, and rapid detection method for  R .  anatipestifer , which is of great importance for the prevention and control of  R .  anatipestifer  infections.

Development of Colloidal Gold Immunochromatographic Strips for Detection of Riemerella anatipestifer